Formation of a centromere-specific chromatin structure
نویسندگان
چکیده
منابع مشابه
CENP-B Controls Centromere Formation Depending on the Chromatin Context
The centromere is a chromatin region that serves as the spindle attachment point and directs accurate inheritance of eukaryotic chromosomes during cell divisions. However, the mechanism by which the centromere assembles and stabilizes at a specific genomic region is not clear. The de novo formation of a human/mammalian artificial chromosome (HAC/MAC) with a functional centromere assembly requir...
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We have taken advantage of the known structural parameters associated with centromere DNA in vivo to construct a CEN fragment that can be selectively excised from the chromatin DNA with restriction endonucleases. CEN3 DNA is organized in chromatin such that a 220-250-bp region encompassing the elements of centromere homology is resistant to nuclease digestion. Restriction enzyme linkers encodin...
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ژورنال
عنوان ژورنال: Epigenetics
سال: 2012
ISSN: 1559-2294,1559-2308
DOI: 10.4161/epi.20389